FastQCFastQC Report
Sun 19 Mar 2023
SRR4895767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162838
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72220.6210667350052199No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT31540.2712329662429332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1165750.0143.826632
GCTTCAG396050.0143.754623
TGGCTTC1168650.0143.469731
GCTTCAT426550.0143.312623
GCTTCAA326950.0142.890523
CTTCATG162550.0142.639164
CTTCAGG146800.0142.423514
CTTCAGT136250.0142.180794
CTTCAGA113100.0141.960434
CTTCATA125700.0140.807564
CTTCAAG112100.0140.075444
CTTCAAT122400.0139.125984
TTCAGTA39450.0136.333185
TTCAGAT40350.0136.329775
TTCATAG46550.0136.137865
TTCATGG53850.0135.623055
TTCAGAG40650.0135.14635
TCAGGAT14250.0135.084696
TCAGATG13400.0135.045046
TTCAGGT49550.0134.588465
Produced by FastQC (version 0.11.5)