FastQCFastQC Report
Sun 19 Mar 2023
SRR4895767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162838
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG324872.793768349503542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA82410.7086971701991163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74810.6433398289357589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22450.1930621462318913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12050.10362578450308642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT646900.0106.097681
AGCAGTG664550.0103.148862
GTGGTAT662300.0103.135666
GGTATCA665200.0103.111078
AGTGGTA664100.0102.915125
TGGTATC664200.0102.906117
GTATCAA667200.0102.812859
CAGTGGT669850.0102.09464
GCAGTGG681550.0100.3419653
CGGAGCC3650.039.724426145
AGCGGTA1504.3992346E-529.0000785
AGGTATC5700.027.9813297
GAGGTAT5700.027.9813296
AGGGGTA4350.025.0000675
ATGGGAG98500.024.67257125-29
ATGGGAT84600.024.66420225-29
CATGGGG113900.023.97194325-29
CATGGGT46450.023.84977525-29
ATGGGGG41500.022.74623725-29
AGAGGTA7350.022.6871365
Produced by FastQC (version 0.11.5)