FastQCFastQC Report
Sun 19 Mar 2023
SRR4895766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530923
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA1536000.0143.711141
GAACAAT556000.0143.113653
GGAACAA1543750.0142.989672
GAACAAG536300.0142.469183
AACAATG199400.0139.489624
AACAAGT198700.0138.92884
GAACAAA449850.0136.849783
ACAATGG73150.0136.50245
ACAAGTA58150.0136.37915
ACAAGTG68550.0135.986535
ACAATGT68250.0135.42235
ACAATTG74050.0135.232795
ACAATAG59250.0134.942265
CAAGTAG21300.0134.035666
AACAAGG204000.0133.057594
ACAATGA56200.0131.874735
ACAAGGT67300.0131.763415
CAATATG22000.0131.737126
ACAAGAT50300.0131.003545
CAATGAG20150.0130.95166
Produced by FastQC (version 0.11.5)