FastQCFastQC Report
Sun 19 Mar 2023
SRR4895766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530923
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG154901.0118079093461918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129530.8460908876540493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24170.1578786130981114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC23100.15088936543510026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18280.11940509091574168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243350.093.607848
GTGGTAT243400.093.231186
AGTGGTA244400.092.998035
AAGCAGT246850.092.9946061
GTATCAA245950.092.759629
TGGTATC247500.091.7160347
AGCAGTG251450.091.059712
CAGTGGT252400.090.171184
GCAGTGG258250.088.156663
CGGTCTG3850.037.661057145
GCTATTC10750.029.673409145
GGCTATC6950.025.035118145
CGATAGG6200.024.5575435-39
TGCGATA6350.023.7492430-34
CATGGGG77750.023.42345625-29
TACGCAC3750.023.20193950-54
CGACCTA3700.022.7304445-49
TCGTAAG7350.022.096506100-104
GGTCGCG8550.022.04862650-54
CGTAAGG7000.021.75125100-104
Produced by FastQC (version 0.11.5)