FastQCFastQC Report
Sun 19 Mar 2023
SRR4895765_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895765_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842514
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG845450.0143.696321
GAAGAGA851450.0142.683722
AAGAGAT299950.0141.218843
AAGAGAG305900.0139.438393
AAGAGAA257100.0133.178393
AGAGAGT110350.0128.583854
AGAGATA95000.0128.261634
GAGATGC7100.0127.951645
GAGAGTC7950.0127.8755
GAGATTG39500.0127.406625
AGAGATG119100.0126.946384
AGAGATT115850.0125.342144
AGAGAAT92650.0124.588964
AGAGAGG128550.0124.1203464
GAGAAGT33150.0123.75495
GAGATAG32000.0123.470795
GAGAGTA34150.0123.2979355
GAGAGAT34650.0123.183395
AGAGAAG106200.0122.610284
AGAGAGA97000.0121.974914
Produced by FastQC (version 0.11.5)