FastQCFastQC Report
Sun 19 Mar 2023
SRR4895765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842514
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99771.184193971850913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG58080.6893653992693296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT9060.10753530505131072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8820.10468668769895813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108900.0100.9967968
GTGGTAT109250.099.8045966
AAGCAGT110850.099.154731
AGTGGTA110450.098.851535
TGGTATC110850.098.6314857
GTATCAA112050.098.0928349
CAGTGGT112650.097.119844
AGCAGTG112900.097.0974352
GCAGTGG114350.095.6125953
CGGTCTG1555.4961583E-528.06695145
GCTATCC3357.4578566E-1125.972403145
TGCGGCT5350.024.39464145
AGGTATC2401.5972018E-624.167337
CGATAGG2150.023.6044635-39
TACGCAC2050.023.34071750-54
CATGGGG36800.022.89383525-29
CTTCGAT1700.003090838421.32538145
TACATGG106500.021.29422820-24
CAACGCA110100.021.20378110-14
CGACCTA2400.021.14515945-49
Produced by FastQC (version 0.11.5)