FastQCFastQC Report
Sun 19 Mar 2023
SRR4895764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501859
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52840.3518306312376861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA1507850.0143.62262
TCTTCAC1510150.0143.403871
TTCACAG522350.0143.202593
TCACAGT189600.0142.865464
TCACATG196950.0142.622274
TCACAGG189100.0141.908774
TTCACAT536900.0141.806093
TCACAAG148700.0141.529684
TCACAGA143050.0141.121954
TCACATA155450.0140.254064
TTCACAA442850.0140.077423
TCACAAT162650.0139.98534
CACATAG54750.0139.587375
CACATGT76750.0139.311555
CACAGTG64700.0139.293275
CACAGTA56550.0139.22415
CACAGTT64400.0138.598715
CACATGG64250.0138.136785
TCACATT185050.0138.079444
CACATGA52900.0138.063285
Produced by FastQC (version 0.11.5)