FastQCFastQC Report
Sun 19 Mar 2023
SRR4895764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501859
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG217091.445475241017965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131640.8765137073453633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35950.23937000743744918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA305050.092.8306668
GTGGTAT305850.092.2322856
AGTGGTA306200.092.1772845
AAGCAGT309250.091.7523351
GTATCAA308650.091.74189
TGGTATC308350.091.508017
CAGTGGT315100.089.720764
AGCAGTG316950.089.402962
GCAGTGG323000.087.4593
CGGTCTG3500.029.000448145
AGCGCAT2054.040885E-728.293118145
CATGGGG82700.023.6705725-29
TACGCAC3150.020.25360550-54
ACATGGG284700.019.76145620-24
TACATGG298700.019.58282520-24
CGACCTA3350.019.47648445-49
CAACGCA309700.019.25523410-14
AGAGTAC309150.019.24766515-19
GTACATG307300.019.24712220-24
CAGAGTA310600.019.19982315-19
Produced by FastQC (version 0.11.5)