FastQCFastQC Report
Sun 19 Mar 2023
SRR4895763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434326
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145861.0169236282407206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG135900.9474833475792812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20920.14585247705193938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA15250.10632171486816805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14910.10395126352028758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221900.094.322868
AAGCAGT223800.094.212211
AGTGGTA223050.093.547275
GTATCAA224300.093.471969
GTGGTAT224150.093.052616
TGGTATC226100.092.314217
AGCAGTG231800.090.7698752
CAGTGGT231650.090.1400764
GCAGTGG236350.088.4396063
CGGTCTG3300.039.54587145
CATGGGG71300.023.59120625-29
CGCTGTT8000.021.75023145
CGCGAAA3100.021.51545790-94
TACGCAC3800.021.36715550-54
CGACCTA4050.021.12220845-49
TACATGG218150.020.12687120-24
AGAGTAC221950.019.99064315-19
ACATGGG212400.019.93443720-24
GTACATG221250.019.9169620-24
CAGAGTA223700.019.8472215-19
Produced by FastQC (version 0.11.5)