FastQCFastQC Report
Sun 19 Mar 2023
SRR4895762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196269
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2096710.682787398926981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29391.4974346432702057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16000.8152076996367231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10070.5130713459588626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG9720.4952386775293092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCA8060.4106608786919992No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5490.2797181419378506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4870.2481288435769276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3980.20278291528463485No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3710.18902628535326516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTCCTGGCAAGTAAAAAAAA3450.17577916023416842No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2970.1513229292450667No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2410.1227906597577814No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2400.12228115494550845No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2360.12024313569641665No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2360.12024313569641665No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2210.11260056351232238No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2200.11209105870004941No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2160.11005303945095762No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2080.105977000952774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGC100.007086252145.002039
CGGTCTG406.649394E-572.501015145
CGTGGGG2250.061.223083145
AAGCAGT162600.046.550471
GTATCAA164700.045.956939
GTGGTAT164600.045.8850676
TGGTATC164750.045.854977
AGTGGTA165450.045.693145
GGTATCA165700.045.6358268
CAGTGGT166700.045.350514
GCAGTGG168500.044.909073
AGCAGTG168950.044.8323552
CGAGCGG855.325306E-542.64766145
CTTGAGA1451.9141225E-840.000565145
CCTGGGG3350.030.298935145
CGTGGGA1702.9951552E-629.853361145
CGCGAAA255.1548827E-428.9989390-94
AGAGAGT3005.7843863E-1026.583706145
CGACCTA507.8487574E-826.09371645-49
CGCGGAC351.2555819E-424.857492135-139
Produced by FastQC (version 0.11.5)