FastQCFastQC Report
Sun 19 Mar 2023
SRR4895761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008870
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41640.4127390050254245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC1009800.0144.076161
ATCAGCA1009800.0144.076162
TCAGCAT358050.0143.929893
TCAGCAG342300.0143.853713
TCAGCAA292750.0143.694583
CAGCATG132550.0143.450244
CAGCAGT127400.0143.249664
CAGCATT119550.0142.643664
CAGCATA104750.0142.559754
CAGCAAG105250.0142.362084
CAGCAAT106150.0142.309834
AGCATGA32900.0142.020165
CAGCAGG121000.0141.887764
AGCAAGT35650.0141.78475
AGCATGT51600.0141.69655
AGCAGTT43800.0140.919315
CAGCAGA94350.0140.849184
AGCAGTA38200.0140.814165
AGCAATG35600.0140.566025
AGCATGG44100.0140.287695
Produced by FastQC (version 0.11.5)