FastQCFastQC Report
Sun 19 Mar 2023
SRR4895761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008870
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG253712.514793779178685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86180.8542230416208232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA40990.4062961531218096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20670.20488269053495495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT251500.073.335861
GGTATCA260300.070.880928
GTATCAA261550.070.763919
GTGGTAT259100.070.761526
AGTGGTA259400.070.651745
CAGTGGT262600.069.901214
AGCAGTG263200.069.8795852
TGGTATC263050.069.80927
GCAGTGG272650.067.324623
AGCGCAT2207.489434E-726.362286145
CGGTCTG3150.025.316162145
GGTCTGA6950.025.034685145
CGCGAAA3000.022.23219590-94
CATGGGG47100.022.04286225-29
CGCAGGT1650.0026016521.96857145
TGCGGCT6950.020.862238145
CCGCGAA3200.020.8426890-94
CGATAGG851.4733814E-920.4709635-39
CTCGTTG3050.020.44299335-39
TATCAGC7450.020.4362051
Produced by FastQC (version 0.11.5)