FastQCFastQC Report
Sun 19 Mar 2023
SRR4895760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347777
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1351400.0143.815751
AGGATGA1351700.0143.783832
GGATGAG423450.0143.149543
GGATGAT504800.0143.091133
GGATGAA412400.0142.806553
GATGAAT160900.0136.900864
GATGATA159950.0136.767324
GATGATG168800.0136.388414
GATGAGA133150.0136.299134
GATGAGT164150.0136.166984
GATGAGG143600.0136.071084
GGATGAC19250.0135.590383
GATGATT197600.0135.484314
GATGAAG141900.0135.313084
GATGAAA131100.0132.270634
ATGATAG54250.0131.711885
ATGAGTG51200.0130.545175
ATGATTG69100.0130.32735
ATGAATG57100.0130.062525
ATGAGGT50700.0129.414965
Produced by FastQC (version 0.11.5)