FastQCFastQC Report
Sun 19 Mar 2023
SRR4895760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347777
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129330.9595801085787932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79570.590379565759024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173950.0104.7761158
GTGGTAT175450.0103.959126
GTATCAA177100.0102.9125069
TGGTATC177550.0102.8157
AGTGGTA178550.0102.397795
AAGCAGT179550.0102.238821
AGCAGTG183450.099.978852
CAGTGGT183150.099.865544
GCAGTGG191350.095.73753
TAGGATG11700.024.78631
TACATGG168700.022.22667720-24
CATGGGG59500.022.22667125-29
GTACATG172350.022.00835420-24
TCAACGC175450.021.96594210-14
CAACGCA175550.021.96168910-14
AGAGTAC174050.021.9346915-19
ATCAACG174700.021.88595210-14
GCAGAGT177450.021.57978215-19
CAGAGTA177550.021.54312715-19
AGTACAT176550.021.51764320-24
Produced by FastQC (version 0.11.5)