FastQCFastQC Report
Sun 19 Mar 2023
SRR4895759_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895759_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396453
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG468300.0143.497133
TGTTCTA1404150.0143.39922
GTTCTAA409150.0142.410233
GTGTTCT1417450.0142.053681
GTTCTAT513400.0141.872883
CTAGTAG16350.0140.221376
TTCTAGA125250.0140.103094
TCTAGAT46800.0139.876535
TTCTAGG172250.0139.209184
TCTATAG54600.0139.17235
TTCTAAG151400.0138.808984
TCTAGAG39050.0138.651255
TCTAGGA43900.0138.277795
TTCTATG192600.0137.976124
TTCTAGT181500.0137.675544
TCTAAGT51600.0137.203965
CTAGATG14950.0136.956316
TCTAGTA51500.0136.490455
TCTATGG67250.0136.256775
TCTAGGT60650.0136.225595
Produced by FastQC (version 0.11.5)