FastQCFastQC Report
Sun 19 Mar 2023
SRR4895759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396453
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG191861.3739094692051934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109230.7821960352407133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32230.23079903154635353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA31790.22764819152524288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG31120.22285032149309716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC29850.2137558514321642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC16800.12030480080604217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT291500.092.824731
GGTATCA293450.091.936138
GTGGTAT292150.091.9233256
GTATCAA295450.091.331789
AGTGGTA295100.091.2255255
CAGTGGT295850.091.046534
AGCAGTG297700.090.773012
TGGTATC296650.090.52897
GCAGTGG304850.088.50133
CGGTCTG3100.042.09595145
CCGCAAG18100.028.038128145
CGACCTA3700.024.68845-49
CATGGGG68600.023.25094225-29
TACGCAC3950.023.12530550-54
CGGAGCC5950.020.713882145
GAAACCG8600.020.2314485-89
AAACCGC8550.020.01058885-89
TACATGG280050.019.93844220-24
ATGGGAG38500.019.9235625-29
ACATGGG268900.019.83235220-24
Produced by FastQC (version 0.11.5)