FastQCFastQC Report
Sun 19 Mar 2023
SRR4895758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27856
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG151.241128E-4144.296236
GTCATTG1250.0144.296235
CATGGTG351.0913936E-11144.296237
TGTCATA3250.0144.296234
GTCATAG1400.0144.296235
TGTCACG650.0144.296234
CATGGAA151.241128E-4144.296237
TCATTCG151.241128E-4144.296236
TCAGGAA351.0913936E-11144.296236
CATTAGA253.7229256E-8144.296237
GTCAGAG600.0144.296235
TCAATGT253.7229256E-8144.296236
TCTGTCA27850.0144.296232
GTCACGT151.241128E-4144.296235
TCAGAGT351.0913936E-11144.296236
TCAGAGA151.241128E-4144.296236
TCATGGT600.0144.296236
GTCAATA1100.0144.296235
CAGGATT151.241128E-4144.296237
GTCAAGG750.0144.296235
Produced by FastQC (version 0.11.5)