FastQCFastQC Report
Sun 19 Mar 2023
SRR4895758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27856
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG452516.244256174612293No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7922.8431935669155655No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1310.47027570361861004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.38770821367030445No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA860.3087306145893165No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG790.28360137851809303No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG680.24411257897759908No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA600.21539345203905802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCGTGGTATCAACGCAG580.20821367030442275No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACTGGACT570.20462377943710508TruSeq Adapter, Index 10 (96% over 29bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG510.1830844342331993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT490.17590465249856405No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT490.17590465249856405No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG470.16872487076392878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC470.16872487076392878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCAGCAACATTATCGAA470.16872487076392878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG470.16872487076392878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG470.16872487076392878No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG460.16513497989661113No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC460.16513497989661113No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA450.16154508902929351No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC420.1507754164273406No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT420.1507754164273406No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA400.14359563469270534No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT400.14359563469270534No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC390.14000574382538772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAAAATCTGCTGCTTTAA390.14000574382538772No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG390.14000574382538772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG390.14000574382538772No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC380.13641585295807007No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA380.13641585295807007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGTGGTAACTTTTCAAAC360.1292360712234348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC330.11846639862148191No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG320.11487650775416428No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA320.11487650775416428No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC320.11487650775416428No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG310.11128661688684664No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA300.10769672601952901No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG290.10410683515221138No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG280.10051694428489374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAT100.007054155145.04
AAGCAGT34200.058.7207571
AGTGGTA34650.057.9581535
GTATCAA35000.057.378579
AGCAGTG35000.057.378572
CAGTGGT35100.057.2151034
GTGGTAT35350.056.810476
GGTATCA35600.056.6151668
TGGTATC35800.056.0963677
GCAGTGG36050.055.5062453
ACAGTCA400.005989547454.3752
AAATATA400.005989547454.375145
CACGGGA1000.005974967529.0145
CGAGGTC200.0060567629.090-94
ATGCCGC200.0060567629.085-89
TACTTAA401.0569074E-525.375135-139
ATGGGGA701.2732926E-1024.85714325-29
TGTGGAG300.001487836924.16666685-89
ATGGGGC502.255876E-623.225-29
CATGGGG2450.022.48979625-29
Produced by FastQC (version 0.11.5)