FastQCFastQC Report
Sun 19 Mar 2023
SRR4895757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603995
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC28070.4647389465144579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA604250.0144.139182
GTCGTAG604450.0144.091491
CGTAGAG192100.0143.872973
CGTAGAT229600.0143.67693
CGTAGAA174600.0143.591323
GTAGATG79150.0141.02234
GTAGATA75650.0140.493534
GTAGAGT68250.0140.407414
GTAGAGG68500.0139.26344
GTAGAAT66800.0139.245444
GTAGAAG62350.0139.00674
GTAGAGA58500.0138.787464
GTAGATT78650.0137.701584
CGTAGAC10300.0135.810153
TAGATGG28600.0134.630345
TAGAGTG22150.0134.11935
TAGATAG24450.0133.299125
GTAGAAA52750.0132.455224
TAGATAC10800.0132.19325
TAGAGTA21450.0132.109165
Produced by FastQC (version 0.11.5)