FastQCFastQC Report
Sun 19 Mar 2023
SRR4895757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603995
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG143852.381642232137683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61911.025008485169579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19440.32185696901464417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCAGGCGCGGTGGCTCA7580.12549772763019562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG1200.084.6152651
GGTATCA154300.081.1895148
GTATCAA155500.080.55639
AAGCAGT158800.079.241191
AGTGGTA158050.079.1320955
GTGGTAT158700.078.7101066
AGCAGTG160900.077.968662
TGGTATC160700.077.7305157
CAGTGGT161600.077.406564
GCAGTGG164600.075.951693
TCGTAGA1650.061.5281872
CGTAGAT953.1313903E-853.4323733
CGGTCTG1401.4122634E-841.430485145
CCGCAAG5150.032.38014145
ATAGGCG1850.022.731157100-104
CGTAGAG1600.00217148322.661053
CATGGGG32750.022.22330325-29
CGGAGCC2003.1001E-421.751005145
GCGATAG2000.021.023882105-109
TACGGTG1350.020.40869125-129
Produced by FastQC (version 0.11.5)