FastQCFastQC Report
Sun 19 Mar 2023
SRR4895756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834484
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT81500.9766514396920731No Hit
GTACGCAATGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10990.13169815119283293No Hit
GTACGCAAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10900.13061964040053495No Hit
GTACGCAAGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA8390.10054117274866864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA835050.0144.11491
TACGCAA835250.0144.080382
ACGCAAT293350.0143.751483
CGCAAGT116700.0143.600634
ACGCAAG299900.0143.473163
CGCAATG105900.0143.469514
CGCAATA86450.0143.38444
ACGCAAA229700.0142.680283
CGCAATT99000.0142.397584
CGCAAAT81750.0142.189774
GCAATAG30850.0141.179895
CGCAAGG107500.0140.998764
GCAATGT37700.0140.775655
CGCAAGA76300.0140.438224
GCAAGTG36200.0140.433795
CGCAAAG89450.0140.268454
GCAAGTT47350.0139.95445
GCAATTG35700.0139.774815
GCAATGA25400.0139.392325
GCAAATG30400.0138.76295
Produced by FastQC (version 0.11.5)