FastQCFastQC Report
Sun 19 Mar 2023
SRR4895756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834484
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG306603.6741267657618364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57860.6933626049151331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA51340.6152304897397673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCAGTGGCTCA12250.14679730228500487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11110.13313616558256358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCTGGGGTGGTGGCTCA9900.11863618715277945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT294700.065.735351
GTGGTAT298850.064.434366
GTATCAA302100.064.149159
GGTATCA302650.064.084328
AGTGGTA300750.064.07555
TGGTATC301500.063.8718457
CAGTGGT306650.062.9608884
AGCAGTG308000.062.826162
GCAGTGG316950.060.9377063
CTACTAG5100.042.64752145
CGTGGGA4900.032.55137145
GTACGCA3950.031.2028661
ACGCAAT1902.0757034E-730.5266443
GAGGTAT3450.029.4206076
TACGCAA4400.028.0116652
TACTAGA4550.027.088203145
AGGTATC3750.027.068587
CGGTCTG1907.1529794E-626.710814145
GTTCGCG850.025.589125105-109
CGACCTA1950.023.793745-49
Produced by FastQC (version 0.11.5)