FastQCFastQC Report
Sun 19 Mar 2023
SRR4895755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016614
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG111441.0961879336700064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47890.47107358348399686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17190.16909072666715194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16220.15954924878075652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC15330.15079469690561018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10970.10790722929253384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT186750.092.7206951
GGTATCA188100.091.8103948
GTGGTAT187400.091.761956
AGTGGTA189050.091.123425
TGGTATC188950.091.090427
GTATCAA190850.090.483039
AGCAGTG192600.089.795892
CAGTGGT193000.089.3843464
GCAGTGG195450.088.18973
CGGTCTG2650.043.76937145
GTGCGAA2603.6379788E-1233.4681932
CCGCAAG9000.030.608162145
CGGAGCC3350.030.295588145
TAGGTCG551.973749E-723.72498745-49
CATGGGG52200.023.55606725-29
GGTGCGA4006.89397E-1021.7532561
CCGCGAA2750.021.61631290-94
CGCGAAA3000.020.78153690-94
TACGCAC2200.020.4298550-54
GGTCTGA6400.020.388662145
Produced by FastQC (version 0.11.5)