FastQCFastQC Report
Sun 19 Mar 2023
SRR4895754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234787
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAACACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2570.10946091563842973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAGC100.0072014984144.227698
CAATTCG202.1844735E-6144.227697
CATTCGG202.1844735E-6144.227697
CATGCTG151.2552374E-4144.227687
GAGAACA235500.0143.768372
AGAACAT82550.0143.528823
GGAGAAC236050.0143.43341
GAACAGT30300.0142.79974
GAACATT30250.0142.797334
AGAACAG81350.0142.632053
GAACATG27850.0142.156194
GAACATA24500.0141.578614
GAACAAT25500.0139.98574
AACAGTG11200.0139.720585
AGAACAA68350.0139.057863
GAACAGG28700.0138.951074
GAACAGA22600.0138.484124
CAGATGG1250.0138.458577
AACATGT9600.0137.467015
AACATTG11450.0137.299735
Produced by FastQC (version 0.11.5)