FastQCFastQC Report
Sun 19 Mar 2023
SRR4895754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234787
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG84423.5955994156405593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23340.9940925178992023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12880.5485823320711964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCTCGGTGGCTCA9920.42251061600514506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCTGTGGCTCACG5110.21764407739781164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCATGGTGGCTCAC4570.19464450757495091No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCTCGGTGGCTCAC4150.17675595326828147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCATGGTGGCTCACG3460.14736761405018164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3330.14183068057430778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGC100.0070891115144.9885145
AAGCAGT78250.077.450721
AGTGGTA79450.076.007195
GGTATCA80050.075.453568
GTATCAA80400.075.0349049
AGCAGTG80850.074.960042
CAGTGGT80950.074.598784
GTGGTAT81350.074.142866
TGGTATC82050.073.43767
GCAGTGG84350.071.5918353
TCTGCTA1600.054.370686145
CTCTGCT3750.040.596783145
GCACTTG1102.431634E-432.95193145
TATGATC950.00472183930.5238932
CGTTCGA605.875336E-1026.581223105-109
TTGAGCG605.875336E-1026.581223100-104
GTGTCTA1701.0285572E-425.586205145
CGTGGGA1450.001224214424.998016145
ACACGGA2100.024.860468125-129
GGCTCGG2100.024.1668135-39
Produced by FastQC (version 0.11.5)