FastQCFastQC Report
Sun 19 Mar 2023
SRR4895753_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895753_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681915
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT8100.11878313279514309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA682450.0144.083162
GCTCGGT683150.0143.935521
TCGGTAG237800.0143.92653
TCGGTAT233500.0143.767073
CGGTAGT85700.0143.694584
TCGGTAA199700.0143.62173
CGGTATG86150.0143.362464
CGGTATA68800.0143.361014
CGGTAAT70350.0142.764564
CGGTAGA59950.0142.756164
CGGTATT78200.0142.447594
CGGTAGG91100.0142.379064
CGGTAAG70750.0141.95744
CGGTAAA59750.0139.372624
TCGGTAC13350.0138.798653
GGTAGTA28250.0136.797995
GGTATGA24200.0133.771735
GGTATAG24900.0132.61715
GGTAGAG17050.0132.358955
GGTAGTG29200.0131.853525
Produced by FastQC (version 0.11.5)