FastQCFastQC Report
Sun 19 Mar 2023
SRR4895752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840237
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG165511.9698013774685001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46380.5519871179203011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA25780.30681819534250454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13790.16412036127902008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10590.12603586845140122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT290750.0101.296361
GGTATCA295150.099.854028
GTGGTAT293850.099.752986
GTATCAA296050.099.501499
AGTGGTA295150.099.4118655
AGCAGTG296600.099.2925342
TGGTATC295900.099.233397
CAGTGGT296850.098.872864
GCAGTGG304350.096.507843
GCTAACG1650.048.3373031
CGGTCTG1807.2759576E-1140.278687145
CTAACGA2052.910383E-1035.366652
CATGGGG68000.024.69173625-29
GAGGTAT2751.967419E-723.7278086
CATGGGT27100.022.47149525-29
ATGGGGT14400.022.1519625-29
ATGGGGC16900.021.8778925-29
GCCCCAA7650.021.797878145
ACATGGG272350.021.26339520-24
ATGGGGA27700.021.25192325-29
Produced by FastQC (version 0.11.5)