FastQCFastQC Report
Sun 19 Mar 2023
SRR4895751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621486
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT103351.6629497687799886No Hit
ACCACTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC46700.75142481085656No Hit
ACCACTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8300.13355087644773977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT622800.0143.913452
CACTGTA182500.0143.870453
CACTGTG240550.0143.686483
CACTGTT184850.0143.133763
ACTGTGA68050.0142.318624
ACTGTGT94450.0142.317324
ACTGTAT56500.0141.928684
ACTGTAG66750.0141.849324
ACTGTAA57250.0141.832824
ACTGTTG82250.0141.33284
CTGTGAG22000.0140.948215
ACTGTTA54000.0140.887534
CTGTTAG22200.0140.652915
CTGTAAG20750.0140.05575
ACTGTGG79350.0140.045614
CTGTATG24550.0139.526265
CTGTAGT21800.0139.264185
CTGTTGA30450.0138.779135
CTGTAGA19550.0137.955385
ACTGTCG5400.0137.548954
Produced by FastQC (version 0.11.5)