FastQCFastQC Report
Sun 19 Mar 2023
SRR4895751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621486
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG455287.325667834834573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA61180.9844147736232193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47410.7628490424563064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37420.6021052767077617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15680.2522985232169349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG11190.18005232619882025No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10100.1625137171231532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8740.14063068194617417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC8370.13467720914067252No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7800.1255056429267916No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG6420.10330079840897462No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG6420.10330079840897462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT334450.049.8618471
GTATCAA339750.048.9947139
GTGGTAT339600.048.9056746
GGTATCA341000.048.8324438
TGGTATC342000.048.5836757
AGCAGTG346450.048.0929262
GCAGTGG349300.047.5552253
AGTGGTA355000.046.8120885
CAGTGGT358300.046.3607034
CGGTCTG1602.0008883E-1145.30873145
CTTGCGA657.3374395E-444.611668145
CGTGGGA5950.041.425125145
CGTGGGG3350.041.11598145
AAATATA14300.037.514362145
CCTGCGG2008.94579E-932.622288145
TTAGCGC200.00607887128.99758740-44
TACGCAC1450.025.99992650-54
CGACCTA1450.025.99825345-49
CAGGCGG7300.023.6369270-74
GATGGGG1851.830613E-423.511557145
Produced by FastQC (version 0.11.5)