FastQCFastQC Report
Sun 19 Mar 2023
SRR4895750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882572
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA883600.0144.043521
CGAGTAA883800.0144.010932
GAGTAAG319150.0143.152983
GAGTAAT315450.0142.911963
GAGTAAA245700.0141.133363
AGTAAGT114250.0139.607594
AGTAAGG121550.0139.528354
AGTAATG119250.0139.498434
AGTAAGA89000.0138.543524
GTAAGTG39350.0136.885045
AGTAATA90400.0135.99914
AGTAAAG96050.0135.956884
AGTAATT115300.0135.58454
GTAAGTA34550.0135.240595
GTAAGGT41900.0134.577625
GTAATGG46750.0134.189255
GTAATAG32950.0133.710625
AGTAAAT92400.0133.289524
GTAATTG40950.0132.769265
GAGTAAC13800.0132.71953
Produced by FastQC (version 0.11.5)