FastQCFastQC Report
Sun 19 Mar 2023
SRR4895750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882572
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG114501.2973445792524576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69110.7830522608920292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16820.19057935216616886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14370.16281957732626914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10740.12168978848184624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA175650.093.701695
AAGCAGT176800.093.666351
GTGGTAT176000.093.345286
GGTATCA177700.092.656278
TGGTATC178150.092.300147
AGCAGTG179700.092.0740662
GTATCAA179250.091.935969
CAGTGGT180350.091.2247544
GCAGTGG182900.090.1511153
CGGTCTG3000.060.41766145
CGAGTAA2350.040.1093142
GCGAGTA2950.036.86711
TACGCAC3250.024.0913450-54
GTCACGA1550.00180755323.3888055
CATGGGG42650.022.37031725-29
CGACCTA3500.021.12772245-49
ATGGGAG22500.020.42876225-29
CGGCGGT3850.020.33868835-39
CGCGAAA2800.020.19561690-94
GCCCCAA11500.020.174244145
Produced by FastQC (version 0.11.5)