FastQCFastQC Report
Sun 19 Mar 2023
SRR4895749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183352
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT1185150.0143.996461
CCACATA1187100.0143.759922
CACATAG417700.0143.680343
CACATAT405050.0142.987143
ACATAGG155500.0142.453374
ACATATG147150.0142.451394
CACATAA346350.0141.800453
ACATAGT145750.0141.791284
CATATGG48950.0141.711235
ACATAAG122250.0140.91244
ACATAGA118650.0140.872944
CATAGGA43450.0140.564475
CATAGGT49200.0140.404925
CATAATG42850.0140.00855
CATAGTG47000.0139.766285
CATATAG42750.0139.661325
CATATTG48000.0139.258095
CATAAGG45050.0139.253595
CATATGA42500.0138.616535
CATAGTA43750.0138.611685
Produced by FastQC (version 0.11.5)