FastQCFastQC Report
Sun 19 Mar 2023
SRR4895749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183352
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG151651.281529080104652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80670.6817075561624943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24870.21016569879461056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12860.10867434203854813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC11940.10089981679162244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT246800.093.770751
GGTATCA245800.093.70988
AGTGGTA245850.093.336855
GTGGTAT247550.092.6626746
GTATCAA249450.092.5139
TGGTATC250700.091.646867
CAGTGGT252600.090.932644
AGCAGTG256200.090.075592
GCAGTGG263500.087.2811663
CGGTCTG4100.042.43837145
CGAGCCG2252.0008883E-1135.443893145
GCTATCC4950.030.757101145
CTACGGA3555.456968E-1226.548885145
CGATAGG3500.026.51432235-39
CTACTAA33200.026.422785145
GGCTATC6250.025.519606145
CTAACGC1100.025.045916120-124
CATGGGG78950.024.5193425-29
TACGCAC2550.024.44977650-54
CGACCTA2400.024.16588645-49
Produced by FastQC (version 0.11.5)