FastQCFastQC Report
Sun 19 Mar 2023
SRR4895748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858976
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT71410.8313387102782849No Hit
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10010.11653410572588757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC860000.0144.018311
ATAGACA860850.0143.876112
TAGACAT317500.0143.603973
TAGACAG292000.0143.52773
AGACATG128950.0142.740684
AGACAGT103400.0142.520944
TAGACAA241350.0142.402013
GACATTG36100.0141.99755
GACAGAT29750.0141.52865
AGACAAT87100.0141.462784
GACAGTG37550.0141.314325
AGACATT104600.0141.299454
GACATGT58200.0140.973535
GACAATG30200.0140.852115
GACATAG31050.0140.711415
AGACATA85300.0140.390884
GACAGGT36150.0140.006155
GACATGA28600.0139.908875
GACATGG39800.0139.665655
GACAGTT33200.0139.416845
Produced by FastQC (version 0.11.5)