FastQCFastQC Report
Sun 19 Mar 2023
SRR4895748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858976
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG232762.70973810676899No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63300.7369239652795887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA39050.45461107178780313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25370.2953516745520247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC17680.20582647245091829No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14170.16496386394963305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11390.13259974667511082No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG9800.11408933427709274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9370.1090833736914652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT221950.072.651251
GTGGTAT228800.070.060136
GGTATCA230200.069.8868
AGTGGTA230050.069.679445
GTATCAA231050.069.62899
TGGTATC231300.069.365577
AGCAGTG232100.069.3219762
CAGTGGT232650.068.908764
GCAGTGG239000.067.1385963
GATAGAC2300.047.2853781
CCGGCTT2303.488458E-828.367924145
ATAGACA4150.026.207882
CGGTCTG2051.290567E-524.754665145
TACGCAC1800.023.3611250-54
ATAGGCG1950.022.308739100-104
GAAACCG5750.022.19131385-89
TCCGGCG1900.022.13107135-39
CATGGGG37950.021.931525-29
CGACCTA2000.021.74899745-49
AAACCGC5900.021.38136585-89
Produced by FastQC (version 0.11.5)