FastQCFastQC Report
Sun 19 Mar 2023
SRR4895747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434156
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG89092.052027381862741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33680.7757580224619722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG26510.6106100111480666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14340.3302960226278112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13530.31163913432038254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8410.19370917366108034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6390.14718211886971502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC4800.1105593381180958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT141400.096.028961
GGTATCA143300.094.969028
GTATCAA143850.094.605929
GTGGTAT143600.094.215226
CAGTGGT143750.094.116914
AGCAGTG144250.094.0311662
TGGTATC144500.093.678597
GCAGTGG145300.093.013113
AGTGGTA145900.092.7299965
CGGTCTG752.549016E-548.330643145
CCGCAAG4250.035.821537145
GGGTATC1907.13993E-626.7121167
AAACCGC5700.024.67520385-89
GCGCAGG5700.024.42138345-49
ATGGGAG21050.023.83240525-29
CATGGGG23300.023.58452625-29
AGGGGTA1851.8276062E-423.5149155
GAAACCG6150.022.869785-89
AAATATA13950.022.866112145
GTTAGCG452.6535863E-522.5553485-89
Produced by FastQC (version 0.11.5)