FastQCFastQC Report
Sun 19 Mar 2023
SRR4895746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434034
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT434350.0144.077471
CTAGTAG152350.0144.05173
ACTAGTA434500.0144.027742
CTAGTAT150000.0143.424623
CTAGTAA124500.0143.267123
TAGTAGA47950.0136.976464
TAGTAAG44550.0136.911154
TAGTAGT57450.0135.664
CTAGTAC9500.0135.08673
TAGTATA51800.0133.754944
TAGTAGG54250.0133.561874
TAGTAAT46950.0133.137284
AGTAGTG17850.0132.884355
TAGTATG54650.0132.584294
AGTAGTA17900.0131.707635
AGTAGAT17700.0130.344565
AGTAATG17100.0128.593775
AGTATGA15000.0128.332355
AGTAGAG15650.0128.069785
AGTAAGT15350.0127.7546545
Produced by FastQC (version 0.11.5)