FastQCFastQC Report
Sun 19 Mar 2023
SRR4895746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434034
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG150203.460558389434929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35220.8114571669500547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23810.5485745356354571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13270.30573641696272646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10860.25021081297778514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT158600.083.480751
AGTGGTA161400.081.6475
AGCAGTG163100.081.116612
CAGTGGT163450.080.676644
GTGGTAT163950.080.367846
GGTATCA164450.080.343978
GTATCAA164700.080.3263249
GCAGTGG166100.079.345853
TGGTATC166650.079.109277
CGGTCTG850.00274181134.11377145
GTCGTAA1000.006074091428.996704145
AGAGAGT10350.025.214525145
CCGCGAA551.972985E-723.72457790-94
CATGGGG26950.023.56698625-29
TCTCGCA1000.023.20110770-74
TACGCAC905.456968E-1222.55299250-54
CGCGAAA604.5649904E-721.74752890-94
ATGGGGC8650.020.6194825-29
CACGCAG4601.7644197E-1020.486805145
ATGGGGG9350.020.31647725-29
Produced by FastQC (version 0.11.5)