FastQCFastQC Report
Sun 19 Mar 2023
SRR4895745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312123
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT14440.4626381266359736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTG151.2557987E-4144.222847
GAATCTG312700.0143.946121
AATCTGA312750.0143.92312
ATCTGAT106450.0143.81643
ATCTGAG111100.0143.508873
ATCTGAA90350.0143.105453
TCTGATA33500.0142.931294
CTGATAG11150.0142.929385
TCTGAGT41250.0142.64954
TCTGATG39150.0142.196734
TCTGAGG38900.0141.998324
TCTGAAG31900.0141.736244
CTGAGTA11300.0141.670245
CTGATGA10550.0141.488775
TCTGATT33750.0141.445244
TCTGAGA30750.0141.174224
CTGAGTG11450.0141.073885
CTGAAGT11150.0140.989175
TCTGAAT32700.0140.914984
CTGATTG10700.0140.853155
Produced by FastQC (version 0.11.5)