FastQCFastQC Report
Sun 19 Mar 2023
SRR4895744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906924
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT907700.0144.08941
TGGCATA908250.0144.002142
GCATAAG92450.0143.670534
GGCATAG297650.0143.65933
GGCATAT330000.0143.473593
GCATATG115900.0143.40774
GGCATAA266550.0143.377883
GCATAGT105000.0142.91174
GCATAGA90850.0142.787844
GCATAGG101300.0142.294574
GCATATA102700.0142.180344
GCATAAT94450.0142.15524
GCATATT111350.0141.690934
CATATGG37800.0140.782785
CATAAGT33000.0140.064835
CATATTG42050.0139.929445
CATATGT40500.0139.765385
CATATAG35050.0139.690465
CATAGGA28300.0139.630115
CATAGGT35050.0139.278995
Produced by FastQC (version 0.11.5)