FastQCFastQC Report
Sun 19 Mar 2023
SRR4895744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906924
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG154131.6994808826318413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72550.7999567769735942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24320.2681591842315343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18270.20145017664104156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12340.13606432292011236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9890.10904993141652444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT217100.092.5144351
GGTATCA218300.091.7581948
AGTGGTA218850.091.361965
GTGGTAT219000.090.9683466
GTATCAA221000.090.8011869
TGGTATC221800.089.950717
CAGTGGT222600.089.762664
AGCAGTG224850.088.961172
GCAGTGG230050.086.8872763
CGGTCTG2057.2759576E-1238.90062145
CCGCAAG10100.034.453835145
CGACCTA2550.025.01871345-49
CATGGGG50200.023.94462825-29
GGTAACA2155.0572294E-420.2316118
TACGCAC3450.020.17408250-54
ATGGGGC19100.019.81364625-29
TACATGG208500.019.73663720-24
ATAGGCG4950.019.627726100-104
ACATGGG200650.019.61270720-24
AGTACAT212700.019.55824720-24
Produced by FastQC (version 0.11.5)