FastQCFastQC Report
Sun 19 Mar 2023
SRR4895743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607307
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT127572.10058504183222No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT13780.22690336189110286No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT13660.22492742550308162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCA608100.0144.016432
CTGAGCC608400.0143.945431
GAGCCAT228550.0143.922233
GAGCCAG199400.0143.482963
AGCCATG95200.0143.221544
GCCATAG21450.0143.197745
AGCCAGT69150.0142.954914
GAGCCAA171150.0142.689533
AGCCATA63900.0142.51364
AGCCAAT63800.0142.397934
GCCATGA18900.0141.917185
AGCCATT68900.0141.903884
GCCAGAT21200.0141.145175
GCCATGT52600.0140.916295
AGCCAGG71600.0140.278754
GCCAGTA20100.0140.260225
AGCCAGA60100.0140.127124
GCCATAT21800.0139.906435
AGCCAAG58050.0139.610444
GCCAATG21750.0139.565055
Produced by FastQC (version 0.11.5)