FastQCFastQC Report
Sun 19 Mar 2023
SRR4895743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607307
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG261174.300460887162505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43430.7151243110980113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43100.7096904860309531No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7100.11690956962458855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT207250.063.280221
AGCAGTG214400.061.034642
AGTGGTA220900.059.0417755
GTGGTAT221550.058.8637056
GGTATCA222700.058.629678
TGGTATC222800.058.5382777
GTATCAA224900.058.249569
CAGTGGT225000.057.9336784
GCAGTGG230800.056.540633
CGGTCTG1252.7207898E-740.592145
CATGCGA1453.492857E-529.99409145
CGGAGCC1606.832952E-527.182144145
TAACCCG300.001495254724.165884115-119
CGTGCGC1500.001495313824.161905145
CGACCTA1500.023.1958145-49
CATGGGG30000.022.96099525-29
CCGTCTT7100.021.439438145
CCGGCTT3757.765266E-921.262478145
TACGCAC1650.021.0884950-54
TTTCCGG2300.018.91741630-34
Produced by FastQC (version 0.11.5)