FastQCFastQC Report
Sun 19 Mar 2023
SRR4895742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561784
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG562250.0144.093111
TCAATGA563200.0143.850052
CAATGAG188600.0143.686083
CAATGAT203450.0143.193483
CAATGAA162700.0142.359863
AATGATG77650.0139.485174
AATGAGT66700.0138.707664
AATGAGG72150.0138.624424
AATGAAG60350.0137.769034
CAATGAC11850.0136.9193
AATGATA64350.0136.489214
AATGAGA54950.0135.95394
AATGAAT61300.0135.045784
ATGAGTG22950.0133.53835
ATGATAG21500.0133.48865
AATGATT69900.0132.151324
ATGAAGT19550.0130.942675
ATGAGTA21500.0130.805425
ATGAGGA21200.0130.615595
ATGATTG23350.0130.324235
Produced by FastQC (version 0.11.5)