FastQCFastQC Report
Sun 19 Mar 2023
SRR4895742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561784
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG248444.4223402588895375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45010.801197613317574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA37980.6760605499622631No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7990.14222548167979154No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6830.12157697620437749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT229900.067.174471
AGTGGTA231900.066.3455
GGTATCA234250.065.8960958
GTGGTAT233850.065.822786
GTATCAA235650.065.406489
AGCAGTG236000.065.2903752
CAGTGGT235900.065.225844
TGGTATC236750.065.01657
GCAGTGG242400.063.357143
CGGTCTG1850.047.029797145
TGCGGCT3100.042.099255145
TTCACGC1000.006070472329.00171145
GCCCCAA4600.025.218878145
AGGGGTA1500.001492947124.168095
CGATAGA851.8189894E-1223.881634105-109
CCGCGAA1400.023.82071190-94
CGCGAAA1350.023.6289290-94
GGTCTGA3203.376772E-822.657585145
CATGGGG32250.022.21018425-29
CGACCTA1700.022.17580245-49
Produced by FastQC (version 0.11.5)