FastQCFastQC Report
Sun 19 Mar 2023
SRR4895741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771738
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27430.35543150654755884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC772300.0144.08611
GCATACA772800.0143.992892
CATACAG268000.0143.552473
CATACAT263250.0142.965683
ATACAGT95000.0141.769534
ATACATG97250.0141.677444
CATACAA230150.0141.597993
ATACAGG94950.0141.008914
TACAGTG31600.0140.547535
ATACAGA79200.0140.374714
ATACAAG84450.0140.185524
TACAGGT31000.0138.848855
TACAGTA29250.0138.52885
TACATGG33400.0138.369775
TACAGAG25950.0138.08575
TACAGAT26850.0137.753525
TACATGT35100.0136.803365
ACAGTAG10650.0136.07436
TACAGGA26950.0135.904745
TACATAG28950.0135.232445
Produced by FastQC (version 0.11.5)