FastQCFastQC Report
Sun 19 Mar 2023
SRR4895741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771738
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG130411.689822193542368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58690.7604912548040915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23380.3029525564375475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9840.12750441211913888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT186000.091.446251
GGTATCA189100.089.793768
AGTGGTA188750.089.72985
GTGGTAT189600.089.2128146
GTATCAA190200.089.154329
AGCAGTG191350.088.781562
TGGTATC191000.088.634817
CAGTGGT193400.087.540574
GCAGTGG199550.084.769963
CGCATAC2250.051.557081
CGGTCTG2252.8145223E-829.000856145
GCCCCAA8050.027.920078145
AGCGCAT1409.990799E-425.893621145
CGACCTA2850.025.43769845-49
GCATACA4600.025.2197692
TACGCAC3000.024.16581350-54
TCCGGCG2900.023.50069435-39
CATGGGG39600.023.39750925-29
CGGAGCC2903.2831122E-722.500664145
TTAGCGC806.91216E-1021.74951440-44
Produced by FastQC (version 0.11.5)