FastQCFastQC Report
Sun 19 Mar 2023
SRR4895740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433370
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20160.4651914068809562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCA434000.0144.002992
TGGTCAG159450.0143.67633
AGTGGTC435000.0143.671951
TGGTCAT138000.0143.487463
TGGTCAA129950.0143.386633
GGTCATG60500.0143.146294
GGTCAGT52500.0142.845474
GGTCATA37700.0142.688814
GGTCAGA45200.0142.464114
GGTCAAT40850.0142.100714
GTCAGGT19950.0141.688845
GGTCAAG52700.0141.482384
GGTCAGG61700.0141.180334
GTCATAG15550.0140.972925
GTCAGTA14800.0140.808415
GTCATGA16100.0140.635915
GTCAAGT15900.0140.590845
GGTCATT39400.0140.558624
GTCAGTG19200.0140.463295
GTCATTG18350.0140.289325
Produced by FastQC (version 0.11.5)