FastQCFastQC Report
Sun 19 Mar 2023
SRR4895740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433370
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG142633.2911830537416065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29950.6910953688534047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27620.6373306874033735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24500.5653367791956065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13130.30297436370768627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6180.14260331818076932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4460.1029143687841798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT184950.091.658281
GTATCAA190150.088.9508069
GGTATCA190550.088.8021248
AGCAGTG191250.088.5252152
GTGGTAT190300.088.499716
CAGTGGT192050.087.7789154
TGGTATC192150.087.760847
AGTGGTA192800.087.399845
GCAGTGG195450.086.177743
CATGCGA951.0280183E-438.15732145
AGGTATC1157.134091E-637.8255167
CCGCAAG3000.031.416191145
AAATATA13400.030.29805145
CCGGCTT1606.8200585E-527.187088145
GTGATAT2507.810377E-826.0996076
AGTGATA2901.1854354E-825.0025045
GAGGTAT1801.5185025E-424.1663026
TGATATC3052.0281732E-823.7701347
CATGGGG24800.023.62224825-29
CGCAGAT5500.023.46328290-94
Produced by FastQC (version 0.11.5)