FastQCFastQC Report
Sun 19 Mar 2023
SRR4895739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423136
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGA423250.0144.163862
CGACTGG423450.0144.095761
ACTGGAT145350.0143.867693
ACTGGAA127150.0143.591143
ACTGGAG144300.0143.365463
CTGGAGT49350.0142.899934
CTGGATG53000.0142.854454
CTGGATT48250.0142.272174
CTGGAAG46050.0142.179374
CTGGATA44550.0141.948944
CTGGAAT42950.0141.864554
CTGGAGA43700.0140.584844
CTGGAGG51400.0139.866064
CTGGAAA39450.0138.365944
ACTGGAC8400.0138.2063
TGGAGTA15500.0135.841195
TGGATGA16400.0135.421375
TGGATTA14950.0133.603825
TGGAGTG16900.0133.548175
TGGATAG16850.0131.804785
Produced by FastQC (version 0.11.5)